The system includes an ultra high fidelity KOD DNA polymerase complexed with two monoclonal antibodies to permit hot start thermocycling, along with specially formulated 2X buffer. KOD Xtreme Hot Start DNA Polymerase quickly and accurately amplifies genomic and phage/plasmid DNA targets up to 24 and 40 kbp, respectively.
Hot start PCR is a modified form of conventional polymerase chain reaction (PCR) that reduces the presence of undesired products and primer dimers due to non-specific DNA amplification at room (or colder) temperatures. Because the results of PCR are so useful, many variations and modifications of the procedure were developed in order to achieve a higher yields, hot start PCR is one of them.
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Review and cite DNA POLYMERASE protocol, troubleshooting and other methodology information | Contact 71975 KOD Xtreme™ Hot Start DNA Polymerase. μl) consisted of 2× KOD Xtreme™ PCR buffer, 2 mM dNTPs, 10 pmol/μl each forward and reverse primer, 1.0 U/μl KOD Xtreme™ Hot Start DNA polymerase, Genomic DNA was isolated from the fungal mycelia using the GF-1 nucleic acid U/µl KOD Xtreme™ Hot Start DNA polymerase, and 100 ng/µl template DNA, The KOD Xtreme™ Hot Start DNA Polymerase* kit is an optimized PCR enzyme for the amplification of long or GC-rich DNA templates, including mouse tail-tips, Thermo Scientific DreamTaq Hot Start DNA Polymerase is an enhanced hot start Taq DNA polymerase that enables higher PCR specificity, sensitivity, and The reaction conditions are compatible with direct addition of primers and KOD Hot Start DNA Polymerase or KOD XL. DNA Polymerase for amplification of the 15 Oct 2013 “Typically DNA polymerases are stratified into high-fidelity and those for routine DNA polymerase from Thermococcus species KOD [kodakaraensis] with Agilent's PfuTurbo Cx Hotstart enzyme “can read through uraci The PCR error ratio of KOD -Plus- is approximately 80 times less than that of Taq DNA polymerase. Effective for the amplification of GC-rich targets. Hotstart 17 May 2018 PCR amplification of DNA templates from plasmids using KOD Xtreme™ Hot Start DNA Polymerase.
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These antibodies can be applied to the "hot start PCR technology" using KOD DNA polymerase. The 3-D structure of DNA polymerase was characterized in 2003 (Fig. 5) (3). KOD Xtreme polymerase is an optimized PCR system for the amplification of long or GC-rich DNA templates.
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KOD Xtreme™ Hot Start DNA Polymerase
KOD Hot Start DNA Polymerase, KOD XL DNA Polymerase, KOD Xtreme™ Hot Start DNA Polymerase, NovaTaq™ DNA Polymerase, NovaTaq™ Hot Start DNA Polymerase, One Step RT-PCR Kit Use of these products is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224;
KOD -Plus- is based on DNA polymerase from the hyperthermophilic Archaeon Thermococcus kodakaraensis KOD1(1)(2) KOD -Plus- exhibits excellent high PCR fidelity and efficiency. The enzyme solution of KOD -Plus- contains two types of anti-KOD DNA polymerase antibodies that inhibit polymerase and 3'→5' exonuclease activity, thus allowing for Hot Start PCR(3). KOD Xtreme Hot Start DNA Polymerase 9 .
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Review and cite DNA POLYMERASE protocol, troubleshooting and other methodology information | Contact 71975 KOD Xtreme™ Hot Start DNA Polymerase. μl) consisted of 2× KOD Xtreme™ PCR buffer, 2 mM dNTPs, 10 pmol/μl each forward and reverse primer, 1.0 U/μl KOD Xtreme™ Hot Start DNA polymerase, Genomic DNA was isolated from the fungal mycelia using the GF-1 nucleic acid U/µl KOD Xtreme™ Hot Start DNA polymerase, and 100 ng/µl template DNA, The KOD Xtreme™ Hot Start DNA Polymerase* kit is an optimized PCR enzyme for the amplification of long or GC-rich DNA templates, including mouse tail-tips, Thermo Scientific DreamTaq Hot Start DNA Polymerase is an enhanced hot start Taq DNA polymerase that enables higher PCR specificity, sensitivity, and The reaction conditions are compatible with direct addition of primers and KOD Hot Start DNA Polymerase or KOD XL. DNA Polymerase for amplification of the 15 Oct 2013 “Typically DNA polymerases are stratified into high-fidelity and those for routine DNA polymerase from Thermococcus species KOD [kodakaraensis] with Agilent's PfuTurbo Cx Hotstart enzyme “can read through uraci The PCR error ratio of KOD -Plus- is approximately 80 times less than that of Taq DNA polymerase.
In parallel with the study of the activities of KOD DNA polymerase, neutralization antibodies for the polymerase and proofreading activities were developed (2). These antibodies can be applied to the "hot start PCR technology" using KOD DNA polymerase.
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KOD Xtreme™ Hot Start DNA Polymerase is an ultra high fidelity DNA polymerase designed for the most challenging PCR applications, including crude sample PCR, amplification of long strand and highly GC-rich DNA …
ready-to-use solution, Ready-to-use 2X mixture, containing KOD Hot Start DNA Polymerase, two monoclonal antibodies, ultrapure deoxynucleotides, and reaction buffer with MgSO4, optimized for convenient high fidelity PCR., suitable for PCR. Sigma-Aldrich. Kod Xtreme Hot Start Dna Polymerase, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 37 PubMed citations. ZERO BIAS - … KOD Hot Start DNA Polymerase is a premixed complex of the high fidelity KOD DNA Polymerase and two monoclonal antibodies that inhibit the DNA polymerase and 3'→5' exonuclease activities at ambient temperatures (1).
KOD Hot Start DNA Polymerase. 1 Product Result. | Match Criteria: Product Name. 71086. High fidelity DNA polymerase designed for accurate PCR amplification of long strand and GC- rich DNA templates for cloning and cDNA amplification applications. Sigma-Aldrich.
KOD Xtreme Hot Start DNA Polymerase successfully amplifies challenging DNA templates with up to 90% GC content. KOD Xtreme Hot Start DNA Polymerase quickly and accurately amplifies genomic and phage/plasmid DNA targets up to 24 and 40 kbp, respectively. KOD Xtreme Hot Start DNA Polymerase successfully amplifies challenging DNA templates with up to 90% GC content. The KOD Xtreme™ Hot Start DNA Polymerase* kit is an optimized PCR system for the amplification of long or GC-rich DNA templates. The unique formulation enables you to amplify directly from minimally processed samples; allowing highly efficient crude sample PCR target amplification. The KOD Xtreme™ Hot Start DNA Polymerase is a ready-to-use reaction or routine PCR amplification, and optimized for difficult targets Efficiently amplifies up to 90% GC-content DNA templates, genomic targets up to 24 kbp and phage/Lambda targets up to 40 kbp. High fidelity DNA polymerase designed for accurate PCR amplification of long strand and GC- rich DNA templates for cloning and cDNA amplification applications.
Hotstart 17 May 2018 PCR amplification of DNA templates from plasmids using KOD Xtreme™ Hot Start DNA Polymerase. 2× Xtreme Buffer 50 μl. dNTPs (2 mM each) Mercury מרקורי חומרים ומכשור למעבדות ולתעשיה | KOD Hot Start Master Mix. KOD XL DNA Polymerase. Item #: MBS71087 KOD Xtreme™ Hot Start DNA Polymerase is an ultra high fidelity DNA polymerase designed for the most challenging PCR applications, including crude sample KOD Xtreme™ Hot Start DNA Polymerase. Produktkod. 71975.